執行效果：

完整程式碼

from tkinter import *
import tkinter.messagebox
import base64
from tkinter import filedialog
from tkinter.scrolledtext import ScrolledText
import subprocess as sub
import threading
import os

top = Tk()
top.title("歡迎關注gudu12306知乎")
path1=os.path.dirname(os.path.abspath(__file__))
# print(path1)
# print(os.environ["Path"])
os.environ["PATH"] += os.pathsep + path1
# print(os.environ["Path"])

#獲取螢幕尺寸以計算佈局引數，使視窗居螢幕中央,其中width和height為介面寬和高
width=700
height=700
screenwidth = top.winfo_screenwidth() 
screenheight = top.winfo_screenheight() 
alignstr = '%dx%d+%d+%d' % (width,height,(screenwidth-width)/2,(screenheight-height)/2)  
top.geometry(alignstr)

#阻止視窗調整大小
top.resizable(0,0)
img=b'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'
#設定視窗圖示
tmp = open("tmp.ico","wb+")
tmp.write(base64.b64decode(img))
tmp.close()
top.iconbitmap("tmp.ico")
os.remove("tmp.ico")

#框架佈局
frame_root=Frame(top)
frame_left=Frame(frame_root)
frame_left.pack(side=LEFT)
# frame_right.pack(side=RIGHT,anchor=N)
frame_root.pack()

#github源地址
tip0_0= Label(frame_left,text='github源地址:https://github.com/soimort/you-get',font = ('楷體',15))
tip0_0.pack(padx=10,anchor=W)
tip0_1= Label(frame_left,text='僅用於學習研究使用，用於非法用途概不負責',15))
tip0_1.pack(padx=10,anchor=W)

#輸入視訊連結
tip1= Label(frame_left,text='請輸入視訊連結：     ',25))
tip1.pack(padx=10,anchor=W)
#視訊連結輸入框
input_url= Entry(frame_left,bg='#F7F3EC')
input_url.pack(ipadx=159,ipady=8,padx=20,anchor=W)

#請選擇儲存位置：
tip2=Label(frame_left,text='請選擇儲存位置(必填！)： ',25))
tip2.pack(padx=10,anchor=W)
#儲存地址輸入框
input_save_address= Entry(frame_left,bg='#F7F3EC')
input_save_address.pack(ipadx=159,anchor=W)

#載入會員cookies：
tip3=Label(frame_left,text='載入會員cookies(下載會員視訊選填!!!)：',bg="red",18))
tip3.pack(padx=10,pady=5,anchor=W)
tip4=Label(frame_left,text='cookies檔案為火狐瀏覽器的cookies.sqlite檔案',18))
tip4.pack(padx=10,pady=2,anchor=W)
#會員cookies輸入框
input_cookies_address= Entry(frame_left,bg='#F7F3EC')
input_cookies_address.pack(ipadx=159,anchor=W)

#瀏覽本地資料夾，選擇儲存位置
def browse_folder():
  #瀏覽選擇本地資料夾
  save_address = filedialog.askdirectory()
  #把獲得路徑，插入儲存地址輸入框（即插入input_save_address輸入框）
  input_save_address.insert(0,save_address)

#瀏覽本地資料夾，選擇儲存位置
def browse_cookies():
  #瀏覽選擇本地資料夾
  cookies_address = filedialog.askopenfilename()
  #把獲得路徑，插入儲存地址輸入框（即插入input_save_address輸入框）
  input_cookies_address.insert(0,cookies_address)

#下載函式
def download():
  tkinter.messagebox.showinfo(title='Hi',message='已經開始下載，耐性等待請勿重複點選')
  #從輸入框獲取視訊連結
  url=input_url.get()
  # 從輸入框獲取儲存地址
  if input_cookies_address.get()=='':
    cookies_address=''
  else:
    cookies_address="-c "+input_cookies_address.get()
  save_address=input_save_address.get()

  cmd = f'you {cookies_address}  -o {save_address}  {url}'
  print(cmd)
  input_url.delete(0,END)
  input_save_address.delete(0,END)

#將cmd結果重定向到tkinterGUI，即將命令列的結果顯示ScrolledText（滾動文字框）控制元件裡
  p = sub.Popen(cmd,stdin=sub.PIPE,stdout=sub.PIPE,stderr=sub.PIPE,shell=True)
  for line in iter(p.stdout.readline,b''):
    stext.insert(END,line.decode('UTF-8'))
    stext.yview_moveto(1)
    if not sub.Popen.poll(p) is None:
      if line == "":
        break
  p.stdout.close()
  # while p.poll() is None:
  #   output=p.stdout.readline().decode('UTF-8')
  #   stext.insert(END,output)
  #   stext.yview_moveto(1)
  # if p.poll()!=0:
  #   errors=p.stderr.read().decode('UTF-8')
  #   stext.insert(END,errors)
  #   stext.yview_moveto(1)

#為避免在下載時tkinter介面卡死，建立執行緒函式
def thread_it(func,*args):
  # 建立
  t = threading.Thread(target=func,args=args)
  # 守護 !!!
  t.setDaemon(True)
  # 啟動
  t.start()

# “瀏覽資料夾”按鈕
browse_folder_button = Button(top,text='瀏覽',15),bg="green",command=lambda :thread_it(browse_folder))
browse_folder_button.place(relx=0.81,rely=0.24,anchor="nw")

#cookies檔案按鈕
browse_folder_cookies = Button(top,command=lambda :thread_it(browse_cookies))
browse_folder_cookies.place(relx=0.81,rely=0.40,anchor="nw")

# “下載”按鈕
download_button = Button(frame_left,text='下載',command=lambda :thread_it(download))
download_button.pack( padx=20,pady=6,anchor=W)

# ScrolledText元件（滾動文字框）
stext = ScrolledText(frame_left,width=60,height=23,background='#F7F3EC')
stext.pack(padx=20,anchor=W)

top.mainloop()

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