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picrust2 Stopping - all 545 input sequences aligned poorly to reference sequences

技術標籤:16spicrust2pythonlinux伺服器centos

在用picrust2做功能預測是時出現

Stopping - all 545 input sequences aligned poorly to reference sequences (--min_align option specified a minimum proportion of 0.8 aligning to reference sequences).

需要對otu.fa取反向
再進行分析

seqtk seq -r otus.fa >otus_rc.fa