使用Python的hashlib模塊提取MD5，網上參考，覺得這個還不錯，可以作為模塊直接使用。

# -*- coding: utf-8 -*-  
  
import hashlib  
import sys  
import os
def md5hex(word):  
    """ MD5加密算法，返回32位小寫16進制符號 """  
    if isinstance(word, unicode):  
        word = word.encode("utf-8")  
    elif not isinstance(word, str):  
        word = str(word)  
    m = hashlib.md5()  
    m.update(word)  
    return m.hexdigest() 
  
  
def md5sum(fname):  
    """ 計算文件的MD5值 """  
    def read_chunks(fh):  
        fh.seek(0)  
        chunk = fh.read(8096)  
        while chunk:  
            yield chunk  
            chunk = fh.read(8096)  
        else: #最後要將遊標放回文件開頭  
            fh.seek(0)  
    m = hashlib.md5()  
    if isinstance(fname, basestring) and os.path.exists(fname):  
        with open(fname, "rb") as fh:  
            for chunk in read_chunks(fh):  
                m.update(chunk)  
    #上傳的文件緩存 或 已打開的文件流  
    elif fname.__class__.__name__ in ["StringIO", "StringO"] or isinstance(fname, file):  
        for chunk in read_chunks(fname):  
            m.update(chunk)  
    else:  
        return ""  
    return m.hexdigest()
if __name__ == "__main__":
    print (md5hex(sys.argv[1]))
    print (md5sum(sys.argv[2]))

Linux上驗證：

Python提取MD5