1.Seurat

轉自：https://cloud.tencent.com/developer/article/1055892

# Initialize the Seurat object with the raw (non-normalized data).  Keep all
# genes expressed in >= 3 cells (~0.1% of the data). Keep all cells with at
# least 200 detected genes
pbmc <- CreateSeuratObject(raw.data = pbmc.data, min.cells = 3
 
, min.genes = 200, 
    project = "10X_PBMC")

這裡的min.genes是對細胞來說的，過濾掉那些檢測到基因個數少於200的細胞。保留>=200的吧。

min.cells=200，包含那些基因，至少在200個細胞裡表達了。